Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells.
Caio Bidueira DenaniAntonio Real-HohnCarlos Alberto Marques de CarvalhoAndre Marco de Oliveira GomesRafael Braga GonçalvesPublished in: Archives of virology (2021)
Lactoferrin is part of the innate immune system, with antiviral activity against numerous DNA and RNA viruses. Rhinoviruses, the leading cause of the common cold, are associated with exacerbation of respiratory illnesses such as asthma. Here, we explored the effect of bovine lactoferrin (BLf) on RV-B14 infectivity. Using different assays, we show that the effect of BLf is strongest during adhesion of the virus to the cell and entry. Tracking the internalisation of BLf and virus revealed a degree of colocalisation, although their interaction was only confirmed in vitro using empty viral particles, indicating a possible additional influence of BLf on other infection steps.
Keyphrases
- chronic obstructive pulmonary disease
- cell cycle arrest
- single cell
- recombinant human
- induced apoptosis
- immune response
- mycobacterium tuberculosis
- sars cov
- lung function
- cell death
- circulating tumor
- cell therapy
- nucleic acid
- single molecule
- endoplasmic reticulum stress
- respiratory tract
- biofilm formation
- staphylococcus aureus
- cystic fibrosis
- allergic rhinitis
- respiratory failure
- bone marrow
- circulating tumor cells