Aptamer-Functionalized Magnetic Ti 3 C 2 Based Nanoplatform for Simultaneous Enrichment and Detection of Exosomes.
Hongyuan CuiTianfang ZhengNana QianXueqi FuAijun LiShu XingXiao-Feng WangPublished in: Small (Weinheim an der Bergstrasse, Germany) (2024)
Exosomes are nanovesicles secreted by cells, which play a crucial role in various pathological processes. Exosomes have shown great promise as tumor biomarkers because of the abundant secretion during tumor formation. The development of a convenient, efficient, and cost-effective method for simultaneously enriching and detecting exosomes is of utmost importance for both basic research and clinical applications. In this study, an aptamer-functionalized magnetic Ti 3 C 2 composite material (Fe 3 O 4 @Ti 3 C 2 @PEI@DSP@aptamer@FAM-ssDNA) is prepared for the simultaneous enrichment and detection of exosomes. CD63 aptamers are utilized to recognize and capture the exosomes, followed by magnetic separation. The exosomes are then released by cleaving the disulfide bonds of DSP. Compared to traditional methods, Fe 3 O 4 @Ti 3 C 2 @PEI@DSP@aptamer@FAM-ssDNA exhibited superior efficiency in enriching exosomes while preserving their structural and functional integrity. Detection of exosome concentration is achieved through the fluorescence quenching of Ti 3 C 2 and the competitive binding between the exosomes and a fluorescently labeled probe. This method exhibited a low detection limit of 4.21 × 10 4 particles mL -1 , a number that is comparable to the state-of-the-art method in the detection of exosomes. The present study demonstrates a method of simultaneous enrichment and detection of exosomes with a high sensitivity, accuracy, specificity, and cost-effectiveness providing significant potential for clinical research and diagnosis.
Keyphrases
- mesenchymal stem cells
- stem cells
- label free
- loop mediated isothermal amplification
- gold nanoparticles
- real time pcr
- sensitive detection
- quantum dots
- risk assessment
- photodynamic therapy
- oxidative stress
- induced apoptosis
- signaling pathway
- drug delivery
- high resolution
- transcription factor
- single molecule
- living cells
- cancer therapy
- tandem mass spectrometry