Chemical Profile, Cytotoxic Activity and Oxidative Stress Reduction of Different Syringa vulgaris L. Extracts.
Daniela HanganuMihaela NiculaeIrina IelciuNeli-Kinga OlahMelania MunteanuRamona BurtescuRăzvan ȘtefanLoredana OlarEmőke PállSanda AndreiDan Cristian VodnarDaniela BenedecIlioara OnigaPublished in: Molecules (Basel, Switzerland) (2021)
Syringa vulgaris L. (common lilac) is one of the most popular ornamental species, but also a promising not comprehensively studied source of bioactive compounds with important therapeutic potential. Our study was designed to characterize the chemical composition and to assess the antioxidant and cytotoxic properties of ethanolic extracts obtained from S. vulgaris L. flowers, leaves, bark, and fruit. The chemical profile of the ethanolic extracts was investigated using chromatographic (HPLC-DAD-ESI+, GC-MS) and spectral (UV-Vis, FT-IR) methods, while the protective effect against free radicals was evaluated in vitro by different chemical assays (DPPH, FRAP, CUPRAC). The cytotoxic activity was tested on two tumoral cell lines, HeLa, B16F10, using the MTT assay. Significant amounts of free or glycosylated chemical components belonging to various therapeutically important structural classes, such as phenyl-propanoids (syringin, acteoside, echinacoside), flavonoids (quercetin, kaempferol derivatives) and secoiridoids (secologanoside, oleuropein, 10-hydroxy oleuropein, demethyloleuropein, syringalactone A, nuzhenide, lingstroside) were obtained for the flowers, leaves and bark extracts, respectively. Furthermore, MTT tests pointed out a significant cytotoxic potential expressed in a non-dose-dependent manner toward the tumoral lines. The performed methods underlined that S. vulgaris extracts, in particular belonging to flowers and leaves, represent valuable sources of compounds with antioxidant and antitumoral potential.
Keyphrases
- oxidative stress
- ms ms
- simultaneous determination
- high throughput
- optical coherence tomography
- magnetic resonance imaging
- mass spectrometry
- drinking water
- computed tomography
- risk assessment
- cell death
- tandem mass spectrometry
- atomic force microscopy
- climate change
- high performance liquid chromatography
- diabetic rats
- high resolution