Integrated routine workflow using next-generation sequencing and a fully-automated platform for the detection of KRAS, NRAS and BRAF mutations in formalin-fixed paraffin embedded samples with poor DNA quality in patients with colorectal carcinoma.

Claire FranczakLudovic DubouisPauline GilsonMarie HussonMarie RouyerJessica DemangeAgnès LerouxJean-Louis MerlinAlexandre Harlé

Published in: PloS one (2019)

Our study shows that an integrated workflow using NGS and Idylla platform allows the determination of KRAS, NRAS and BRAF mutational statuses of 651/669 (97.3%) samples and retrieve 49/67 (73.1%)samples that don't reach DNA quality requirements for NGS.

Keyphrases

wild type
circulating tumor
high throughput
cell free
single molecule
machine learning
electronic health record
quality improvement
deep learning
gene expression
circulating tumor cells
nucleic acid
solid phase extraction
copy number
mass spectrometry
metastatic colorectal cancer
high resolution
real time pcr