Login / Signup

Mechanoregulation of clathrin-mediated endocytosis.

Joshua P FergusonScott D HuberNathan M WillyEsra AygünSevde GokerTugba AtabeyComert Kural
Published in: Journal of cell science (2017)
We characterized the tension response of clathrin-mediated endocytosis by using various cell manipulation methodologies. Elevated tension in a cell hinders clathrin-mediated endocytosis through inhibition of de novo coat initiation, elongation of clathrin coat lifetimes and reduction of high-magnitude growth rates. Actin machinery supplies an inward pulling force necessary for internalization of clathrin coats under high tension. These findings suggest that the physical cues cells receive from their microenvironment are major determinants of clathrin-mediated endocytic activity.
Keyphrases
  • single cell
  • cell therapy
  • stem cells
  • induced apoptosis
  • signaling pathway
  • cell proliferation
  • bone marrow
  • cell cycle arrest