hVFL3/CCDC61 is a component of mother centriole subdistal appendages required for centrosome cohesion and positioning.

Together, our results support that hVFL3 is required for anchoring MT at SAP during interphase and ensuring proper centrosome cohesion and positioning. The role of the VFL3 family of proteins thus appears to have been conserved in evolution despite the great variation in the shape of centriole appendages in different eukaryotic species.