Role of flow-cytometric immunophenotyping in prediction of BCR/ABL1 gene rearrangement in adult B-cell acute lymphoblastic leukemia.
Francesco CorrenteSilvia BellesiElisabetta MetafuniPier Luigi PuggioniSara MariettiAngela Maria CiminelloTommaso ZaFederica SoràLuana FianchiSimona SicaValerio De StefanoPatrizia ChiusoloPublished in: Cytometry. Part B, Clinical cytometry (2017)
We performed a retrospective analysis of 88 adult patients with B-ALL diagnosed in our center by a flow-cytometric assessment. Immunophenotypic expression of leukemic cells was explored by simultaneous evaluation of positivity, percentage of expressing cells and median fluorescence intensity (MFI). BCR/ABL1 fusion transcripts were assessed by RT-PCR analysis and were identified in 36 patients (40.9%). CD10 and CD34 were positive in the totality of BCR/ABL1-positive cases. Patients with gene rearrangement had a greater frequency of CD66c, CD13 and CD33 positivity compared with BCR/ABL1-negative cases. Moreover, BCR/ABL1-positive cases exhibited a greater median percentage and MFI values of CD13, CD33, CD66c, CD10, CD34 and CD25 expressions, but a lower median percentage and MFI values of CD38 and CD22 expressions than patients without gene rearrangement. Multivariate logistic regression analysis showed that CD10, CD38 and CD13 expressions were independent predictors for the presence of BCR/ABL1 rearrangement. Predictive probabilities of molecular occurrence based on these markers are proposed. © 2017 International Clinical Cytometry Society.
Keyphrases
- acute lymphoblastic leukemia
- tyrosine kinase
- chronic myeloid leukemia
- nk cells
- end stage renal disease
- chronic kidney disease
- newly diagnosed
- risk assessment
- induced apoptosis
- peritoneal dialysis
- acute myeloid leukemia
- genome wide
- cell proliferation
- prognostic factors
- gene expression
- copy number
- transcription factor
- cell death
- cell cycle arrest
- quantum dots
- single molecule
- binding protein
- endoplasmic reticulum stress
- allogeneic hematopoietic stem cell transplantation
- high intensity
- wild type