Discovery and implementation of a novel pathway for n-butanol production via 2-oxoglutarate.
Sofia FerreiraRui PereiraFilipe LiuPaulo VilaçaIsabel RochaPublished in: Biotechnology for biofuels (2019)
In this work, we were able to successfully translate the computational analysis into in vivo applications, designing novel strains of E. coli able to produce n-butanol via an innovative pathway. Our results demonstrate that enumeration algorithms can broad the spectrum of butanol producing pathways. This validation encourages further research to other target compounds.