Cuttlefish bone powder as an efficient solid-phase extraction sorbent of anti-SARS-CoV-2 drugs in environmental water.
Ines TliliRafika BensghaierLatifa LatrousAdel MegrichePublished in: Chemicke zvesti (2022)
Many antiviral drugs were developed to counteract coronavirus disease, 2019 (COVID-19) with severe acute respiratory syndrome. Therefore, the scientific community's efforts have focused on the detection and quantification of antiviral compounds currently being tested for COVID-19 treatment. Cuttlefish bone powder (CFBP) has been used for the first time as solid-phase extraction (SPE) sorbent for the extraction of SARS CoV-2 antiviral drugs (chloroquine, ritonavir and indomethacin) from water samples. An effective and sensitive method was developed by combining SPE and liquid chromatography- UV detection (LC-UV). An experimental design was applied for the optimization of extraction process. Experimental variables were optimized using Doehlert matrix. The developed method included 50 mg of CFBP sorbent, 20 mL of water sample at pH = 9 and 5 mL of ACN/KH 2 PO 4 buffer solution (80:20, v/v) in the elution step. For validation of the method, selectivity, linearity precision, and sensitivity were evaluated. Extraction recovery percentage of all Sars cov-2 antivirals were above 98.2%. The detection and quantification limits were between 0.1 and 0.5 µg L -1 and 0.6 and 2 µg L -1 , respectively. The current study suggested that CFBP has the application potential for the enhanced SPE of SARS CoV-2 antiviral drugs from water samples.
Keyphrases
- solid phase extraction
- sars cov
- liquid chromatography
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- respiratory syndrome coronavirus
- tandem mass spectrometry
- molecularly imprinted
- simultaneous determination
- coronavirus disease
- gas chromatography mass spectrometry
- mass spectrometry
- ultra high performance liquid chromatography
- high resolution mass spectrometry
- gas chromatography
- loop mediated isothermal amplification
- real time pcr
- bone mineral density
- label free
- healthcare
- high resolution
- mental health
- postmenopausal women
- human health
- bone loss
- quality improvement
- risk assessment
- body composition
- bone regeneration
- soft tissue
- quantum dots
- replacement therapy