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Monitoring casbene synthase in Jatropha curcas tissues using targeted proteomics.

Natália Pinto de AlmeidaDomingos Ferreira Mélo NetoGabriel Reis Alves CarneiroAndreza Raquel Barbosa de FariasGilberto Barbosa DomontFrancisco de Assis de Paiva CamposFábio César Sousa Nogueira
Published in: Plant methods (2021)
Our targeted proteomics methods were able to detect and quantify, for the first time, CS gene products and demonstrate the distribution of CS isoforms only in roots from J. curcas genotypes with a high and low concentration of PE. These methods can be expanded to monitor CS, at the protein level, in different tissues and genotypes of J. curcas.
Keyphrases
  • mass spectrometry
  • gene expression
  • cancer therapy
  • label free
  • genome wide
  • copy number
  • drug delivery
  • amino acid
  • binding protein
  • dna methylation
  • small molecule