Impact of Bariatric Surgery on the Stability of the Genetic Material, Oxidation, and Repair of DNA and Telomere Lengths.
Franziska FerkMiroslav MišíkBenjamin ErnstGerhard PragerChristoph BichlerDoris MejriChristopher GernerAndrea BileckMichael KundiSabine LangieKlaus HolzmannSiegfried KnasmuellerPublished in: Antioxidants (Basel, Switzerland) (2023)
Obesity causes genetic instability, which plays a key-role in the etiology of cancer and aging. We investigated the impact of bariatric surgery (BS) on DNA repair, oxidative DNA damage, telomere lengths, alterations of antioxidant enzymes and, selected proteins which reflect inflammation. The study was realized with BS patients ( n = 35). DNA damage, base oxidation, BER, and NER were measured before and 1 month and 6 months after surgery with the single-cell gel electrophoresis technique. SOD and GPx were quantified spectrophotometrically, malondealdehyde (MDA) was quantified by HPLC. Telomere lengths were determined with qPCR, and plasma proteome profiling was performed with high-resolution mass spectrophotometry. Six months after the operations, reduction of body weight by 27.5% was observed. DNA damage decreased after this period, this effect was paralleled by reduced formation of oxidized DNA bases, a decline in the MDA levels and of BER and NER, and an increase in the telomere lengths. The activities of antioxidant enzymes were not altered. Clear downregulation of certain proteins (CRP, SAA1) which reflect inflammation and cancer risks was observed. Our findings show that BS causes reduced oxidative damage of DNA bases, possibly as a consequence of reduction of inflammation and lipid peroxidation, and indicate that the surgery has beneficial long-term health effects.
Keyphrases
- dna damage
- oxidative stress
- dna repair
- bariatric surgery
- circulating tumor
- weight loss
- body weight
- papillary thyroid
- cell free
- single cell
- single molecule
- high resolution
- end stage renal disease
- squamous cell
- obese patients
- chronic kidney disease
- metabolic syndrome
- hydrogen peroxide
- breast cancer cells
- type diabetes
- minimally invasive
- newly diagnosed
- insulin resistance
- mass spectrometry
- rna seq
- genome wide
- prognostic factors
- ejection fraction
- gene expression
- signaling pathway
- high throughput
- patient reported outcomes
- risk assessment
- cell proliferation
- lymph node metastasis
- cell cycle arrest
- body mass index
- anti inflammatory
- childhood cancer
- acute coronary syndrome
- high fat diet induced
- physical activity
- hyaluronic acid
- surgical site infection