Analysis of early cellular responses of anterior cruciate ligament fibroblasts seeded on different molecular weight polycaprolactone films functionalized by a bioactive poly(sodium styrene sulfonate) polymer.
Amélie LerouxJagadeesh K VenkatesanDavid G CastnerMagali CucchiariniVéronique MigonneyPublished in: Biointerphases (2019)
With the growing number of anterior cruciate ligament (ACL) ruptures and the increased interest for regenerative medicine procedures, many studies are now concentrated on developing bioactive and biodegradable synthetic ligaments. For this application, the choice of raw materials with appropriate physicochemical characteristics and long-term degradation features is essential. Polycaprolactone (PCL) has the advantage of slow degradation that depends on its molecular weight. This study evaluates two PCL materials: a technical grade (PC60: 60 kDa) versus a medical grade (PC12: 80 kDa), both before and after functionalization with poly(sodium styrene sulfonate) (pNaSS). After determining the grafting process had little to no effect on the PCL physicochemical properties, sheep ACL fibroblast responses were investigated. The PC12 films induced a significantly lower expression of the tumor necrosis factor alpha inflammatory gene compared to the PC60 films. Both film types induced an overproduction of fibroblast growth factor-2 and transforming growth factor beta compared to the controls on day 5 and demonstrated collagen gene expression profiles similar to the controls on day 7. Upon protein adsorption, pNaSS grafting caused a rapid cell adhesion in the first 30 min and an increased adhesion strength (1.5-fold higher). Moreover, after 7 days, an increase in cell density and actin network development were noted on the grafted films.
Keyphrases
- anterior cruciate ligament
- room temperature
- transforming growth factor
- cell adhesion
- tissue engineering
- high glucose
- diabetic rats
- epithelial mesenchymal transition
- copy number
- carbon nanotubes
- healthcare
- genome wide
- single cell
- drug delivery
- heat shock protein
- rheumatoid arthritis
- binding protein
- drug induced
- dna methylation
- cell migration
- small molecule
- ionic liquid
- genome wide identification
- stem cells
- aqueous solution
- staphylococcus aureus
- escherichia coli
- high resolution
- protein protein
- reduced graphene oxide
- extracellular matrix
- mesenchymal stem cells
- genome wide analysis
- molecularly imprinted
- liquid chromatography