Transcriptional dynamics of tail regeneration in Xenopus tropicalis.
Jessica ChangJulie BakerAndrea Elizabeth WillsPublished in: Genesis (New York, N.Y. : 2000) (2017)
In contrast to humans, many amphibians are able to rapidly and completely regenerate complex tissues, including entire appendages. Following tail amputation, Xenopus tropicalis tadpoles quickly regenerate muscle, spinal cord, cartilage, vasculature and skin, all properly patterned in three dimensions. To better understand the molecular basis of this regenerative competence, we performed a transcriptional analysis of the first 72 h of tail regeneration using RNA-Seq. Our analysis refines the windows during which many key biological signaling processes act in regeneration, including embryonic patterning signals, immune responses, bioelectrical signaling and apoptosis. Our work provides a deep database for researchers interested in appendage regeneration, and points to new avenues for further study.
Keyphrases
- stem cells
- rna seq
- spinal cord
- wound healing
- single cell
- gene expression
- immune response
- transcription factor
- oxidative stress
- cell free
- mesenchymal stem cells
- skeletal muscle
- spinal cord injury
- cell death
- emergency department
- neuropathic pain
- heat shock
- cell proliferation
- computed tomography
- soft tissue
- toll like receptor
- data analysis