Antioxidant Activities of an Exopolysaccharide (DeinoPol) Produced by the Extreme Radiation-Resistant Bacterium Deinococcus radiodurans.
Shun Mei LinChan Yu BaekJong-Hyun JungWoo Sik KimHa-Yeon SongJi Hee LeeHyun Jung JiYong ZhiBo Sun KangYong-Sun BahnHo Seong SeoSangyong LimPublished in: Scientific reports (2020)
Deinococcus radiodurans shows extreme resistance to a range of remarkable environmental stresses. Deinococcal exopolysaccharide (DeinoPol) is a component of the cell wall, but its role in stress resistance has not yet been well-described. In this study, we isolated and characterized DeinoPol from Deinococcus radiodurans R1 strain and investigated its application as an antioxidant agent. Bioinformatic analysis indicated that dra0033, encoding an ExoP-like protein, was involved in DeinoPol biosynthesis, and dra0033 mutation significantly decreased survival rates in response to stresses. Purified DeinoPol consists of different monosaccharides and has a molecular weight of approximately 80 to 100 kDa. DeinoPol also demonstrates highly protective effects on human keratinocytes in response to stress-induced apoptosis by effectively scavenging ROS. Taken together, these findings indicate that DeinoPol is the first reported deinococcal exopolysaccharide that might be used in cosmetics and pharmaceuticals as a safe and attractive radical scavenger.
Keyphrases
- cell wall
- induced apoptosis
- oxidative stress
- endoplasmic reticulum stress
- endothelial cells
- signaling pathway
- climate change
- anti inflammatory
- dna damage
- cell death
- stress induced
- heat shock protein
- induced pluripotent stem cells
- radiation therapy
- wound healing
- high resolution
- atomic force microscopy
- single molecule
- risk assessment
- data analysis