Drug resistance pattern among ART-naive clients attending an HIV testing and counseling center in Dhaka, Bangladesh.
Sezanur RahmanMd S SarkerShambhu G AralaguppeGolam SarwarSharful I KhanMustafizur RahmanPublished in: Journal of medical virology (2021)
In Bangladesh, antiretroviral therapy (ART) is provided without screening drug resistance-associated mutations (DRM) among people living with HIV, while DRM might emerge and transmit to the newly infected individual. The present study was aimed to identify DRM among ART-naive clients from an HIV testing and counseling (HTC) center in the initial stages of ART programs. Randomly selected (n = 64) archived plasma samples were used for the pol gene amplification and sequencing by sanger technology. Recovered sequences (n = 10) were genotyped using HIV genotyping tools of NCBI and analyzed using the Stanford University HIV drug resistance database (hivdb.stanford.edu). Various genotypes with a number of DRM were identified in HTC clients, who belonged to different risk groups based on behavioral data. The drug resistance algorithm showed that all samples were fully resistant to tipranavir/ritonavir drugs except for one intermediate resistance. Despite the small sample size, our understanding from this study warrants an ART policy with a DRM monitoring system for the country.
Keyphrases
- hiv testing
- antiretroviral therapy
- hiv infected
- men who have sex with men
- human immunodeficiency virus
- hiv positive
- hiv infected patients
- hiv aids
- public health
- machine learning
- genome wide
- emergency department
- big data
- south africa
- single cell
- dna methylation
- transcription factor
- aortic dissection
- smoking cessation
- gene expression
- tertiary care
- nucleic acid
- neural network