Determination of Calystegines in Several Tomato Varieties Based on GC-Q-Orbitrap Analysis and Their Classification by ANOVA.

In this study, several calystegines (A3, A5, B1, B2, B3, B4, and C1) were determined in tomato. A simple extraction followed by a derivatization step with silylating agents was performed prior to their analysis by gas chromatography coupled to high resolution mass spectrometry (GC-HRMS-Q-Orbitrap), which allowed the monitoring of several ions at accurate mass. The validation of the method has provided suitable values of linearity, trueness (73.7-120.0%), and precision (≤20.0%, except for calystegines B3 and B4 at 0.5 mg/kg). The limit of quantitation was set at 0.5 mg/kg for all analytes. The validated method was successfully applied to the analysis of nine different tomato varieties, and calystegines A3, A5, B2, and C1 were found at concentrations ranging between 0.65 mg/kg (C1) and 12.47 mg/kg (B2). Tomato varieties were classified according to their calystegines content by applying an analysis of variance (ANOVA).