Inkjet Printing Based Separation of Mammalian Cells by Capillary Electrophoresis.
Weifei ZhangNan LiHulie ZengHizuru NakajimaJin-Ming LinKatsumi UchiyamaPublished in: Analytical chemistry (2017)
This study describes a method to investigate the separation of cells by capillary electrophoresis (CE) coupled with inkjet printing system. The results validated the feasibility of inkjet printing for mammalian cells to achieve the drop-on-demand and convenient sampling into capillary then zone electrophoresis was applied to separate different cells according to their electrophoretic mobility, finally the peak signal were measured by UV detector. Linear relationship between the peak area and the droplet number was obtained within the range of 25-400 drops (R2 = 0.996) at a fixed cell concentration 106/mL, indicating that this system could be used for rapid and accurate quantification of cells.
Keyphrases
- capillary electrophoresis
- induced apoptosis
- cell cycle arrest
- mass spectrometry
- endoplasmic reticulum stress
- single cell
- magnetic resonance imaging
- cell death
- high resolution
- stem cells
- liquid chromatography
- oxidative stress
- signaling pathway
- magnetic resonance
- computed tomography
- mesenchymal stem cells
- bone marrow
- pi k akt
- contrast enhanced
- sensitive detection