Non-viral vaccination through cationic guanidium polymer-pDNA polyplex mediated gene transfer.
David C LutherRitabrita GoswamiYi-Wei LeeTaewon JeonRui HuangJames L EliaHarini NagarajJetta J E BijlsmaMartin PiestMartijn A LangereisVincent M RotelloPublished in: Nanoscale (2023)
Vaccination through cellular transfection of nucleotide-based vaccines is a powerful approach to combatting disease. Plasmid DNA (pDNA) vaccines are particularly promising vectors for non-viral immunomodulation that afford high degrees of potency and flexibility. Versatile guanidinium-functionalized poly(oxanorbornene)imide (PONI-Guan) homopolymers were used to facilitate non-disruptive pDNA condensation into discrete polyplexes, enabling efficient in vitro transfection of endothelial cells and HD-11 macrophages. Translation of these vectors for vaccination of white leghorn chickens against Newcastle disease virus (NDV) elicited strong humoral immune responses against the virus. This approach presents a highly versatile method for targeted immunomodulation in vivo , with the potential for translatability as a non-viral vaccine platform.
Keyphrases
- disease virus
- immune response
- sars cov
- endothelial cells
- escherichia coli
- circulating tumor
- crispr cas
- high throughput
- genome wide
- dna methylation
- high resolution
- toll like receptor
- high glucose
- risk assessment
- quantum dots
- molecularly imprinted
- nucleic acid
- heat stress
- drug delivery
- climate change
- transcription factor
- vascular endothelial growth factor