EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling.
Dorothee HölzlGeorg HutarewBarbara ZellingerBeate Alinger-ScharingerHans U SchlickerChristoph SchwartzKarl SotlarTheo F J KrausPublished in: Biomedicines (2022)
Gliomas are the most common intrinsic brain tumors in adults, and in accordance with their clinical behavior and patients' outcome, they are graded by the World Health Organization (WHO) classification of brain tumors. One very interesting candidate for targeted tumor therapy may be epidermal growth factor receptor ( EGFR ) amplification. Here, we performed an integrated comparative analysis of EGFR amplification in 34 glioma samples using standard fluorescence in situ hybridization (FISH) and Illumina EPIC Infinium Methylation Bead Chip and correlated results with molecular glioma hallmarks. We found that the EPIC analysis showed the same power of detecting EGFR amplification compared with FISH. EGFR amplification was detectable in high-grade gliomas (25%). Moreover, EGFR amplification was found to be present solely in IDH wildtype gliomas (26%) and TERT mutated gliomas (27%), occurring independently of MGMT promoter methylation status and being mutually exclusive with 1p/19q codeletion (LOH). In summary, EPIC Bead Chip analysis is a reliable tool for detecting EGFR amplification and is comparable with the standard method FISH. EGFR amplification is a phenomenon of IDH wildtype TERT mutated high-grade gliomas.
Keyphrases
- high grade
- epidermal growth factor receptor
- low grade
- tyrosine kinase
- nucleic acid
- small cell lung cancer
- advanced non small cell lung cancer
- dna methylation
- single molecule
- label free
- end stage renal disease
- chronic kidney disease
- wild type
- gene expression
- machine learning
- deep learning
- transcription factor
- high throughput
- newly diagnosed
- single cell
- bone marrow
- quantum dots
- mesenchymal stem cells
- cell free
- cell therapy
- energy transfer