NIR-II Protein-escaping Dyes Enable High-contrast and Long-term Prognosis Evaluation of Flap Transplantation.
Yijing DuJiajun XuXue ZhengZetao DangNingning ZhuZijian JiangJia LiShoujun ZhuPublished in: Advanced materials (Deerfield Beach, Fla.) (2023)
Real-time vascular positioning, postoperative flap monitoring, and vascular reconstruction assessment are of great importance in flap transplantation. Cyanine dyes offer the advantage of high resolution in the NIR-II imaging window. However, the non-specific binding of many cyanine dyes to endogenous albumin leads to high organ accumulation and skin absorption, resulting in low-quality imaging and poor reproducibility of contrast during long-term (e.g., 7 days) postoperative monitoring. Here, we propose a novel strategy that can be widely applied to prevent protein binding for NIR-I/II Cl-containing cyanine dyes. This strategy produces protein-escaping dyes, ensuring high fluorescence enhancement in the blood with rapid clearance and no residual fluorescence, allowing for short-term repeatable injections for vascular imaging. We have successfully applied this strategy in the perioperative monitoring of pedicle perforator flap models in mice and rats. Furthermore, leveraging the universality of this strategy, we design and synthesize multiple non-overlapping protein-escaping probes that achieve dual-excitation (808 nm and 1064 nm) interference-free imaging of nerve-vessel and tumor-vessel simultaneously. These protein-escaping dyes enable long-term repeatable dual-color imaging of tumor localization, resection, and tumor-vessel reconstruction at the wound site. This article is protected by copyright. All rights reserved.
Keyphrases
- high resolution
- photodynamic therapy
- fluorescence imaging
- patients undergoing
- protein protein
- binding protein
- soft tissue
- magnetic resonance
- amino acid
- breast reconstruction
- magnetic resonance imaging
- type diabetes
- mesenchymal stem cells
- metabolic syndrome
- drug delivery
- skeletal muscle
- adipose tissue
- energy transfer
- high speed
- robot assisted
- sensitive detection
- dna binding
- quantum dots
- ultrasound guided