Probing Ca2+-induced conformational change of calmodulin with gold nanoparticle-decorated single-walled carbon nanotube field-effect transistors.
Wenting ShaoSeth C BurkertDavid L WhiteValerie L ScottJianfu DingZhao LiJianying OuyangFrançois LapointePatrick R L MalenfantKabirul IslamAlexander StarPublished in: Nanoscale (2019)
Nanomaterials are ideal for electrochemical biosensors, with their nanoscale dimensions enabling the sensitive probing of biomolecular interactions. In this study, we compare field-effect transistors (FET) comprised of unsorted (un-) and semiconducting-enriched (sc-) single-walled carbon nanotubes (SWCNTs). un-SWCNTs have both metallic and semiconducting SWCNTs in the ensemble, while sc-SWCNTs have a >99.9% purity of semiconducting nanotubes. Both SWCNT FET devices were decorated with gold nanoparticles (AuNPs) and were then employed in investigating the Ca2+-induced conformational change of calmodulin (CaM) - a vital process in calcium signal transduction in the human body. Different biosensing behavior was observed from FET characteristics of the two types of SWCNTs, with sc-SWCNT FET devices displaying better sensing performance with a dynamic range from 10-15 M to 10-13 M Ca2+, and a lower limit of detection at 10-15 M Ca2+.
Keyphrases
- protein kinase
- gold nanoparticles
- single molecule
- molecular dynamics simulations
- label free
- high glucose
- reduced graphene oxide
- carbon nanotubes
- walled carbon nanotubes
- endothelial cells
- diabetic rats
- molecular dynamics
- quantum dots
- drug induced
- oxidative stress
- atomic force microscopy
- machine learning
- convolutional neural network
- deep learning