Dialysis Preparation of Smart Redox and Acidity Dual Responsive Tea Polyphenol Functionalized Calcium Phosphate Nanospheres as Anticancer Drug Carriers.
Xiuli RenPeng ZhangZhenhua ChenPublished in: Molecules (Basel, Switzerland) (2020)
Large-scale preparation of biocompatible drug delivery systems with targeted recognition and controlled release properties has always been attractive. However, this strategy has been constrained by a lot of design challenges, such as complicated steps and premature drug release. Herein, in this paper, we address these problems by a facile in situ mineralization method, which synthesizes biodegradable tea polyphenol coated monodisperse calcium phosphate nanospheres using for targeted and controlled delivery of doxorubicin. Dialysis diffusion method was used to control ion release to form mineralized nanospheres. The polyphenol coatings and calcium phosphate used in this work could be biodegraded by intracellular glutathione and acidic microenvironment, respectively, resulting the release of encapsulated drug. According to confocal fluorescence microscopy, and cytotoxicity experiments, the prepared tea polyphenol functionalized, doxorubicin loaded calcium phosphate nanospheres were confirmed to have highly efficient internalization and obvious cell killing effect on target tumor cells, but not normal cells. Our results suggest that these tea polyphenols functionalized calcium phosphate nanospheres are promising vehicles for controlled release of an anticancer drug in cancer therapy.
Keyphrases
- cancer therapy
- drug delivery
- drug release
- highly efficient
- molecularly imprinted
- quantum dots
- chronic kidney disease
- mental health
- single molecule
- induced apoptosis
- end stage renal disease
- optical coherence tomography
- stem cells
- single cell
- ionic liquid
- emergency department
- cell therapy
- cell cycle arrest
- mesenchymal stem cells
- cell proliferation
- reactive oxygen species
- endoplasmic reticulum stress
- energy transfer
- mass spectrometry
- electronic health record
- bone regeneration