A Novel, Reverse Transcription, Droplet Digital PCR Assay for the Combined, Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus 2 with Swine Acute Diarrhea Syndrome Coronavirus.

Zhou Zhang Na Wang Xiaofei Liu Jizhou Lv Hongli Jing Xiangfen Yuan Dongjie Chen Xiangmei Lin Shaoqiang Wu

Published in: Journal of AOAC International (2022)

This RT-ddPCR assay could be used to detect both SARS-CoV-2 and SADS-CoV in a sample with one double-quenched probe, and is also the first reported RT-ddPCR assay for SADS-CoV detection.

Keyphrases

respiratory syndrome coronavirus
sars cov
sensitive detection
high throughput
coronavirus disease
quantum dots
loop mediated isothermal amplification
liver failure
transcription factor
respiratory failure
case report
aortic dissection