A multispectral-sensor-based colorimetric reader for biological assays.
M PirbhaiClaire AlbrechtC TirrellPublished in: The Review of scientific instruments (2021)
Tests that depend on changes in color are commonly used in biosensing. Here, we report on a colorimetric reader for such applications. The device is simple to construct and operate, making it ideal for research laboratories with limited resources or skilled personnel. It consists of a commercial multispectral sensor interfaced with a Raspberry Pi and a touchscreen. Unlike camera-based readers, this instrument requires no calibration of wavelengths by the user or extensive image processing to obtain results. We demonstrate its potential for colorimetric biosensing by applying it to the birefringent enzyme-linked immunosorbent assay. It was able to prevent certain false positives that the assay is susceptible to and lowered its limit of detection for glucose by an order of magnitude.
Keyphrases
- gold nanoparticles
- label free
- high throughput
- hydrogen peroxide
- fluorescent probe
- sensitive detection
- living cells
- fluorescence imaging
- aqueous solution
- loop mediated isothermal amplification
- deep learning
- nitric oxide
- blood glucose
- type diabetes
- machine learning
- blood pressure
- single molecule
- convolutional neural network
- low cost
- metabolic syndrome
- quantum dots
- insulin resistance