Reliable differentiation of Pneumocystis pneumonia from Pneumocystis colonisation by quantification of Major Surface Glycoprotein gene using real-time polymerase chain reaction.
Shivaprakash Mandya RudramurthyMegha SharmaMadhubala SharmaPankaj RawatAnup GhoshLakshmishree VenkatesanRitesh AggarwalMeenu SinghArunoloke ChakrabartiPublished in: Mycoses (2017)
A clear differentiation between pneumonia due to Pneumocystis jirovecii and "colonisation" is required for optimal case management. A quantification of fungal burden using major surface glycoprotein (MSG) gene-based real-time PCR was undertaken for the same. Lower respiratory tract samples collected from 104 patients of clinically suspected Pneumocystis pneumonia (PCP) were subjected to quantitative PCR using MSG gene. Based on whether or not the cases were treated for PCP, the efficacy of qPCR to differentiate between "diseased" and "colonised" was evaluated. Standard curve of plasmid-cloned gene and receiver operating characteristic curve defined a cut-off of Ct ≤ 25 to diagnose PCP and Ct within 26-39.3 range to depict colonisation. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the qPCR was 100%, each for diagnosing PCP. MSG-gene-based qPCR is a robust tool for the reliable differentiation of Pneumocystis pneumonia from colonisation.
Keyphrases
- genome wide
- copy number
- end stage renal disease
- genome wide identification
- respiratory tract
- chronic kidney disease
- real time pcr
- computed tomography
- magnetic resonance imaging
- dna methylation
- ejection fraction
- peritoneal dialysis
- magnetic resonance
- prognostic factors
- crispr cas
- transcription factor
- intensive care unit
- community acquired pneumonia
- genome wide analysis
- acute respiratory distress syndrome
- mass spectrometry
- pet ct
- structural basis
- patient reported