Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract.
Fu SunAnurup GanguliJudy NguyenRyan BrisbinKrithika ShanmugamDavid L HirschbergMatthew B WheelerRashid BashirDavid M NashBrian T CunninghamPublished in: Lab on a chip (2020)
Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.
Keyphrases
- loop mediated isothermal amplification
- nucleic acid
- infectious diseases
- sensitive detection
- high throughput
- chronic rhinosinusitis
- circulating tumor cells
- coronavirus disease
- gram negative
- endothelial cells
- sars cov
- public health
- antimicrobial resistance
- single cell
- real time pcr
- oxidative stress
- multidrug resistant
- adverse drug
- emergency department
- anti inflammatory
- quantum dots
- respiratory syndrome coronavirus
- genetic diversity