Single-Cell Imaging of mRNA by Target RNA-Initiated RCA.
Xiaojun RenYifan WuRuijie DengJinghong LiPublished in: Methods in molecular biology (Clifton, N.J.) (2024)
We present a powerful method for direct mRNA detection based on ligation-based recognition and in situ amplification, capable of single-cell imaging mRNA at single-nucleotide and single-molecule resolution. Attributed to the use of Splint R ligase that can ligate padlock probe with RNA as target template, this method can efficiently detect mRNA in the absence of reverse transcription. This method enables spatial localization and correlation analysis of gene expression in single cells, which helps us to elucidate gene function and regulatory mechanisms.
Keyphrases
- single molecule
- single cell
- gene expression
- living cells
- high resolution
- rna seq
- binding protein
- nucleic acid
- transcription factor
- high throughput
- induced apoptosis
- dna methylation
- atomic force microscopy
- genome wide
- copy number
- cell cycle arrest
- quantum dots
- cell death
- oxidative stress
- endoplasmic reticulum stress
- molecularly imprinted
- loop mediated isothermal amplification
- photodynamic therapy