Variety and Dynamics of Proteoforms in the Human Proteome: Aspects of Markers for Hepatocellular Carcinoma.
Stanislav NaryzhnyVictor ZgodaArthur T KopylovElena PetrenkoOlga KleistАlexander ArchakovPublished in: Proteomes (2017)
We have previously developed an approach, where two-dimensional gel electrophoresis (2DE) was used, followed by sectional analysis of the whole gel using high-resolution nano-liquid chromatography-mass spectrometry (ESI LC-MS/MS). In this study, we applied this approach on the panoramic analysis of proteins and their proteoforms from normal (liver) and cancer (HepG2) cells. This allowed us to detect, in a single proteome, about 20,000 proteoforms coded by more than 4000 genes. A set of 3D-graphs showing distribution of these proteoforms in 2DE maps (profiles) was generated. A comparative analysis of these profiles between normal and cancer cells showed high variability and dynamics of many proteins. Among these proteins, there are some well-known features like alpha-fetoprotein (FETA) or glypican-3 (GPC3) and potential hepatocellular carcinoma (HCC) markers. More detailed information about their proteoforms could be used for generation of panels of more specific biomarkers.
Keyphrases
- mass spectrometry
- liquid chromatography
- high resolution
- tandem mass spectrometry
- high resolution mass spectrometry
- ms ms
- endothelial cells
- high performance liquid chromatography
- gas chromatography
- healthcare
- capillary electrophoresis
- simultaneous determination
- hyaluronic acid
- gene expression
- climate change
- squamous cell
- induced pluripotent stem cells
- bioinformatics analysis