Preparation and in Vitro Evaluation of Tamoxifen-Conjugated, Eco-Friendly, Agar-Based Hybrid Magnetic Nanoparticles for Their Potential Use in Breast Cancer Treatment.
Zanib KhanSadia SattarMuhammad AbubakarMuhammad Javed ArshedRoohi AslamSyed Tahir Abbas ShahSundus JavedAamira TariqShumaila ManzoorNazish BostanPublished in: ACS omega (2023)
Tamoxifen is the drug of choice as hormonal therapy for hormone receptor-positive breast cancers and can reduce the risk of breast cancer recurrence. However, oral tamoxifen has a low bioavailability due to liver and intestinal metabolic passes. To overcome this problem and utilize the potential of this drug to its maximum, inorganic nanoparticle carriers have been exploited and tested to increase its bioavailability. Biocompatibility and unique magnetic properties make iron oxide nanoparticles an excellent choice as a drug delivery system. In this study, we developed and tested a "green synthesis" approach to synthesize iron nanoparticles from green tea extract and coated them with agar for longer stability (AG-INPs). Later, these hybrid nanoparticles were conjugated with tamoxifen (TMX). By using this approach, we synthesized stable agar-coated tamoxifen-conjugated iron nanoparticles (TMX-AG-INPs) and characterized them with Fourier-transform infrared (FTIR) spectroscopy. The average particle size of AG-INPs was 26.8 nm, while the average particle size of tamoxifen-loaded iron nanoparticles, TMX-AG-INPs, was 32.1 nm, as measured by transmission and scanning electron microscopy. The entrapment efficiency of TMX-AG-INPs obtained by the drug release profile was 88%, with a drug loading capacity of 43.5%. TMX-AG-INPs were significantly ( p < 0.001) efficient in killing breast cancer cells when tested in vitro on the established breast cancer cell line MCF-7 by cell viability assay, indicating their potential to control cell proliferation.
Keyphrases
- breast cancer cells
- quantum dots
- photodynamic therapy
- electron microscopy
- estrogen receptor
- positive breast cancer
- highly efficient
- drug release
- cell proliferation
- visible light
- drug delivery
- magnetic nanoparticles
- high resolution
- oxidative stress
- molecularly imprinted
- emergency department
- type diabetes
- human health
- metabolic syndrome
- walled carbon nanotubes
- high throughput
- decision making
- skeletal muscle
- climate change
- insulin resistance
- polycystic ovary syndrome
- pi k akt
- single cell
- low cost
- free survival
- wound healing
- light emitting