The need for housing is an important concern for most women about to be released from prison, especially by those that need substance use treatment. This study explored the association between housing settings, relationships, and substance use. Two hundred adult females that had endorsed previous incarceration within the past two years participated in the initial baseline study. This study used multi-level modeling to analyze if housing settings and/or relationships within a living condition are associated with substance use. Results indicated that participants in homeless, and mutual settings used substances significantly more than participants in their own house or apartment. Participants that were in controlled, residential and transitional settings used significantly less than those living in their own house or apartment. Living with parents, family, or sexual partner significantly increased substance use. Utilizing these results to plan housing arrangements before women are released from correctional facilities may be beneficial for substance use recovery. Currently, there is no literature that directly measures the relationship between distinct housing settings, as well as the relationships that exist within them and substance use.