Svalbamides A and B, Pyrrolidinone-Bearing Lipodipeptides from Arctic Paenibacillus sp.
Young Eun DuEun Seo BaeYeonjung LimJang-Cheon ChoSang-Jip NamJongheon ShinSang-Kook LeeSeung-Il NamDong-Chan OhPublished in: Marine drugs (2021)
Two new secondary metabolites, svalbamides A (1) and B (2), were isolated from a culture extract of Paenibacillus sp. SVB7 that was isolated from surface sediment from a core (HH17-1085) taken in the Svalbard archipelago in the Arctic Ocean. The combinational analysis of HR-MS and NMR spectroscopic data revealed the structures of 1 and 2 as being lipopeptides bearing 3-amino-2-pyrrolidinone, d-valine, and 3-hydroxy-8-methyldecanoic acid. The absolute configurations of the amino acid residues in svalbamides A and B were determined using the advanced Marfey's method, in which the hydrolysates of 1 and 2 were derivatized with l- and d- forms of 1-fluoro-2,4-dinitrophenyl-5-alanine amide (FDAA). The absolute configurations of 1 and 2 were completely assigned by deducing the stereochemistry of 3-hydroxy-8-methyldecanoic acid based on DP4 calculations. Svalbamides A and B induced quinone reductase activity in Hepa1c1c7 murine hepatoma cells, indicating that they represent chemotypes with a potential for functioning as chemopreventive agents.
Keyphrases
- ms ms
- climate change
- amino acid
- induced apoptosis
- high resolution
- mass spectrometry
- magnetic resonance
- multiple sclerosis
- oxidative stress
- cell cycle arrest
- molecular docking
- high glucose
- density functional theory
- heavy metals
- cell proliferation
- molecular dynamics simulations
- molecular dynamics
- big data
- risk assessment