Development of a quadruple qRT-PCR assay for simultaneous identification of hypervirulent and carbapenem-resistant Klebsiella pneumoniae .
Zhixiang XuBaisheng LiYushan JiangJia HuangLebin SuWeibo WuQilin PangZhuolin LiJiaqi ZhangXiaohe LiJun WangFulan CenLing PengJinhu LiangFuxiang WangChang LiuChenguang ShenYing-Xia LiuYang YangPublished in: Microbiology spectrum (2023)
(hvKp) and carbapenem-resistant Kp (CR-Kp) infections poses a huge public health challenge with high morbidity and mortality. Worrisomely, due to the mobility of elements carrying virulence and drug-resistance genes, the increasing prevalence of CR-hvKp has also been found with an overwhelming mortality rate in recent years. However, the current detection methods for hvKp and CR-Kp have many disadvantages, such as long turnaround time, complex operation, low sensitivity, and specificity. Herein, a more sensitive, rapid, single-reaction, and multiplex quantitative real-time PCR was developed and validated to differentiate the circulating lineages of Kp with excellent performance in sensitivity and specificity, providing a useful tool for the differential diagnosis and the surveillance of the circulating Kp.
Keyphrases
- real time pcr
- klebsiella pneumoniae
- public health
- escherichia coli
- multidrug resistant
- risk factors
- high throughput
- staphylococcus aureus
- pseudomonas aeruginosa
- loop mediated isothermal amplification
- helicobacter pylori
- type diabetes
- helicobacter pylori infection
- biofilm formation
- cardiovascular events
- coronary artery disease
- transcription factor
- mass spectrometry
- single cell
- cystic fibrosis
- sensitive detection