Genotoxic and antiproliferative properties of Endopleura uchi bark aqueous extract.
Glaucia Cristina de Lima E Souza MesquitaElkejer Ribeiro Da CruzDione Silva CorrêaAlexandre de Barros Falcão FerrazJéssica Machado MiriIngrid Vicente FariasFlávio Henrique ReginattoFernanda Brião Menezes BoarettoDuani Maria Dos SantosJuliana da SilvaIvana GrivicichJaqueline Nascimento PicadaPublished in: Journal of toxicology and environmental health. Part A (2024)
The bark extract from Endopleura uchi has been widely used in traditional medicine to treat gynecological-related disorders, diabetes, and dyslipidemias albeit without scientific proof. In addition, E. uchi bark extract safety, especially regarding mutagenic activities, is not known. The aim of this study was to determine the chemical composition, antitumor, and toxicological parameters attributed to an E. uchi bark aqueous extract. The phytochemical constitution was assessed by colorimetric and chromatographic analyzes. The antiproliferative effect was determined using sulforhodamine B (SRB) assay using 4 cancer cell lines. Cytotoxic and genotoxic activities were assessed utilizing MTT and comet assays, respectively, while mutagenicity was determined through micronucleus and Salmonella /microsome assays. The chromatographic analysis detected predominantly the presence of gallic acid and isoquercitrin. The antiproliferative effect was more pronounced in human colon adenocarcinoma (HT-29) and human breast cancer (MCF-7) cell lines. In the MTT assay, the extract presented an IC 50 = 39.1 µg/ml and exhibited genotoxic (comet assay) and mutagenic (micronucleus test) activities at 20 and 40 µg/ml in mouse fibroblast cell line (L929) and mutagenicity in the TA102 and TA97a strains in the absence of S9 mix. Data demonstrated that E. uchi bark possesses bioactive compounds which exert cytotoxic and genotoxic effects that might be associated with its antitumor potential. Therefore, E. uchi bark aqueous extract consumption needs to be approached with caution in therapeutic applications.
Keyphrases
- oxidative stress
- high throughput
- endothelial cells
- anti inflammatory
- escherichia coli
- ionic liquid
- squamous cell carcinoma
- machine learning
- young adults
- radiation therapy
- skeletal muscle
- induced pluripotent stem cells
- high resolution
- adipose tissue
- climate change
- deep learning
- insulin resistance
- mass spectrometry
- squamous cell
- breast cancer cells
- living cells
- lymph node metastasis