Quantification of Protein Palmitoylation by Cysteine-SILAC.
Caiyun FangXiaoqin ZhangHao-Jie LuPublished in: Methods in molecular biology (Clifton, N.J.) (2022)
Cysteine-SILAC enables the detection and quantification of protein S-palmitoylation, an important protein posttranslational modification. Here we describe the cell culture, protein extraction, selective enrichment, mass spectrometry, and data analysis for palmitoylated proteins from cell samples by this method.