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Protein-Protein Communication Mediated by an Antibody-Responsive DNA Nanodevice.

Simona RanalloDaniela SorrentinoElisabetta DelibatoGianfranco ErcolaniKevin W PlaxcoFrancesco Ricci
Published in: Angewandte Chemie (International ed. in English) (2022)
We report here the rational design and optimization of an antibody-responsive, DNA-based device that enables communication between pairs of otherwise non-interacting proteins. The device is designed to recognize and bind a specific antibody and, in response, undergo a conformational change that leads to the release of a DNA strand, termed the "translator," that regulates the activity of a downstream target protein. As proof of principle, we demonstrate antibody-induced control of the proteins thrombin and Taq DNA polymerase. The resulting strategy is versatile and, in principle, can be easily adapted to control protein-protein communication in artificial regulatory networks.
Keyphrases
  • protein protein
  • small molecule
  • single molecule
  • circulating tumor
  • cell free
  • cancer therapy
  • nucleic acid
  • transcription factor
  • molecular dynamics
  • molecular dynamics simulations
  • circulating tumor cells