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Improved results of LINE-1 methylation analysis in formalin-fixed, paraffin-embedded tissues with the application of a heating step during the DNA extraction process.

Xianyu WenSeorin JeongYounghoon KimJeong Mo BaeNam Yun ChoJung Ho KimGyeong Hoon Kang
Published in: Clinical epigenetics (2017)
Formalin fixation leads to artificial increases in the measured values of LINE-1 methylation, and the application of prolonged heating of DNA samples decreases the discrepancy in the measured values of LINE-1 methylation between paired FF and FFPE tissue samples. The application of prolonged heating of DNA samples improves bisulfite conversion-based measurement of LINE-1 or single gene methylation levels in FFPE tissue samples.
Keyphrases
  • genome wide
  • circulating tumor
  • dna methylation
  • cell free
  • single molecule
  • gene expression
  • nucleic acid
  • minimally invasive
  • genome wide analysis