Detection of rare carbapenemases in Enterobacterales-comparison of two colorimetric and three CIM-based carbapenemase assays.
Lukas SchaffarczykJanina NosterYvonne StelzerJanko SattlerSören GatermannAxel G HamprechtPublished in: Microbiology spectrum (2024)
Detection of so-called rare carbapenemases (e.g., GES, IMI, OXA-23, and OXA-58) in Enterobacterales is challenging, and data on the performance of currently available assays are scarce. This study systematically assessed the performance of currently recommended and novel hydrolysis-based assays on a set of molecularly characterized isolates. It demonstrates that the currently recommended assays mCIM and Carba NP perform well on isolates producing common carbapenemases such as KPC, VIM, NDM, and OXA-48, but have only a moderate sensitivity in the detection of rare carbapenemases. In contrast, the newer CIM-based variants, sCIM and mzCIM, are equally capable of detecting frequent and uncommon carbapenemases. These assays could potentially help to improve our knowledge on the epidemiology of these "rare" enzymes.
Keyphrases
- klebsiella pneumoniae
- multidrug resistant
- high throughput
- escherichia coli
- loop mediated isothermal amplification
- label free
- real time pcr
- healthcare
- gold nanoparticles
- acinetobacter baumannii
- magnetic resonance
- magnetic resonance imaging
- gene expression
- risk factors
- dna methylation
- high intensity
- hydrogen peroxide
- copy number
- electronic health record
- nitric oxide
- pseudomonas aeruginosa
- cystic fibrosis
- single cell
- aqueous solution