Identification of antigenic proteins in Strongyloides stercoralis by proteomic analysis.
Rutchanee RodpaiPewpan M IntapanTongjit ThanchomnangOranuch SanpoolPenchom JanwanPorntip LaummaunwaiChaisiri WongkhamTonkla InsawangWanchai MaleewongPublished in: Parasitology research (2017)
Strongyloides stercoralis is an intestinal helminth that infects people worldwide. Hyperinfection or disseminated human strongyloidiasis can involve vital organs, leading to lethal outcomes. We analyzed immunoproteomics of antigenic spots, derived from S. stercoralis third-stage larvae and reacted with human strongyloidiasis sera, by two-dimensional gel electrophoresis and immunoblotting. Of 26 excised antigenic spots analyzed by liquid chromatography-electrospray ionization-tandem mass spectrometry, 20 proteins were identified. Most proteins were associated with enzymes involved in the metabolic process, energy generation, and oxidation-reduction. The proteins relate to promotion of worm development, cell division, cell signaling and transportation, and regulation of muscular contraction. Identification of antigenic proteins shows promise in helping to discover potential diagnostic protein markers or vaccine candidates for S. stercoralis infection.
Keyphrases
- tandem mass spectrometry
- liquid chromatography
- endothelial cells
- ultra high performance liquid chromatography
- simultaneous determination
- mass spectrometry
- high performance liquid chromatography
- single cell
- gas chromatography
- high resolution mass spectrometry
- type diabetes
- stem cells
- high resolution
- small molecule
- hydrogen peroxide
- nitric oxide
- machine learning
- solid phase extraction
- body composition
- bioinformatics analysis
- deep learning
- insulin resistance
- big data
- bone marrow
- binding protein