Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere.
Jônatas AbrahãoLorena SilvaLudmila Santos SilvaJacques Yaacoub Bou KhalilRodrigo RodriguesThalita ArantesFelipe AssisPaulo BorattoMiguel AndradeErna Geessien KroonBergmann Morais RibeiroIvan BergierHerve SeligmannEric GhigoPhilippe ColsonAnthony LevasseurGuido KroemerDidier RaoultBernard La ScolaPublished in: Nature communications (2018)
Here we report the discovery of two Tupanvirus strains, the longest tailed Mimiviridae members isolated in amoebae. Their genomes are 1.44-1.51 Mb linear double-strand DNA coding for 1276-1425 predicted proteins. Tupanviruses share the same ancestors with mimivirus lineages and these giant viruses present the largest translational apparatus within the known virosphere, with up to 70 tRNA, 20 aaRS, 11 factors for all translation steps, and factors related to tRNA/mRNA maturation and ribosome protein modification. Moreover, two sequences with significant similarity to intronic regions of 18 S rRNA genes are encoded by the tupanviruses and highly expressed. In this translation-associated gene set, only the ribosome is lacking. At high multiplicity of infections, tupanvirus is also cytotoxic and causes a severe shutdown of ribosomal RNA and a progressive degradation of the nucleus in host and non-host cells. The analysis of tupanviruses constitutes a new step toward understanding the evolution of giant viruses.
Keyphrases
- genome wide
- induced apoptosis
- rare case
- genome wide identification
- escherichia coli
- multiple sclerosis
- small molecule
- cell cycle arrest
- binding protein
- genetic diversity
- copy number
- early onset
- single molecule
- high throughput
- signaling pathway
- dna methylation
- cell death
- cell proliferation
- pi k akt
- neural network
- bioinformatics analysis
- quality control