Repair effect of centella asiastica (L.) extract on damaged HaCaT cells studied by atomic force microscopy.

People's choice of cosmetics is no longer just "Follow the trend", but pays more attention to the ingredients of cosmetics, whether the ingredients of cosmetics are beneficial to people's skin health, therefore, more and more skin-healthy ingredients have been discovered and used in cosmetics. In this work, atomic force microscope (AFM) is used to provide physical information about biomolecules and living cells, it brings us a new method of high-precision physical measurement. Centella asiastica (L.) extract has the ability to promote skin wound healing, but its healing effect on damaged HaCaT cells needs to be investigated, which plays a key role in judging the effectiveness of skincare ingredients. The objective of this study was to explore the impact of Centella asiastica (L.) extract on ethanol-damaged human immortalized epidermal HaCaT cells based on AFM. We established a model of cellular damage and evaluated cell viability using the MTT assay. The physical changes of cell height, roughness, adhesion and Young's modulus were measured by AFM. The findings indicated that the Centella asiastica (L.) extract had a good repair effect on injured HaCaT cells, and the optimal concentration was 75 μg/ml. Lay Description In the beauty world, choosing cosmetics is not just about following fashion but prioritizing the benefits of cosmetics on your skin health is more important. This study employs cutting-edge technology called Atomic Force Microscopy (AFM) to explore deep into the effectiveness of skincare ingredients. The ingredient under study through atomic force microscope is Centella asiatica extract, which is a natural compound gaining attention for its ability to promote skin wound healing. However, to truly understand its skincare potential, it is crucial to investigate its ability to repair damaged HaCaT cells, which plays a pivotal role in determining skincare product efficacy. The primary objective of this study is to utilize AFM to uncover how Centella asiatica extract can repair ethanol-damaged HaCaT cells. The results are promising. Centella asiatica extract demonstrates a clear ability to repair damaged HaCaT cells, offering a hope for improved skincare. This research underscores that selecting cosmetics goes beyond merely keeping up with fashion trends but comprehending how ingredients like Centella asiatica extract can genuinely benefit your skin health. Thanks to the precision of AFM technology, we now have a more profound insight into how these ingredients work at the cellular level, instilling greater confidence in our skincare choices. This study provides valuable scientific insights for those in the field of skincare and beauty products. This article is protected by copyright. All rights reserved.