Acute Neuropixels Recordings in the Marmoset Monkey.
Nicholas M DotsonZachary W DavisPatrick JendritzaJohn H ReynoldsPublished in: eNeuro (2024)
High-density linear probes, such as Neuropixels, provide an unprecedented opportunity to understand how neural populations within specific laminar compartments contribute to behavior. Marmoset monkeys, unlike macaque monkeys, have a lissencephalic (smooth) cortex that enables recording perpendicular to the cortical surface, thus making them an ideal animal model for studying laminar computations. Here we present a method for acute Neuropixels recordings in the common marmoset ( Callithrix jacchus ). The approach replaces the native dura with an artificial silicon-based dura that grants visual access to the cortical surface, which is helpful in avoiding blood vessels, ensures perpendicular penetrations, and could be used in conjunction with optical imaging or optogenetic techniques. The chamber housing the artificial dura is simple to maintain with minimal risk of infection and could be combined with semichronic microdrives and wireless recording hardware. This technique enables repeated acute penetrations over a period of several months. With occasional removal of tissue growth on the pial surface, recordings can be performed for a year or more. The approach is fully compatible with Neuropixels probes, enabling the recording of hundreds of single neurons distributed throughout the cortical column.
Keyphrases
- liver failure
- high density
- respiratory failure
- drug induced
- high resolution
- aortic dissection
- small molecule
- fluorescence imaging
- spinal cord
- hepatitis b virus
- living cells
- single molecule
- spinal cord injury
- extracorporeal membrane oxygenation
- mental health
- photodynamic therapy
- liquid chromatography
- acute respiratory distress syndrome
- fluorescent probe
- mechanical ventilation
- tandem mass spectrometry