Detection and Diversity of Fungal Nitric Oxide Reductase Genes (p450nor) in Agricultural Soils.

Steven A HigginsAllana WelshLuis H OrellanaKonstantinos T KonstantinidisJoanne C Chee-SanfordRobert A SanfordChristopher W SchadtFrank E Löffler

Published in: Applied and environmental microbiology (2016)

A comprehensive understanding of the microbiota controlling soil N loss and greenhouse gas (N2O) emissions is crucial for sustainable agricultural practices and addressing climate change concerns. We report the design and application of a novel PCR primer set targeting fungal p450nor, a biomarker for fungal N2O production, and demonstrate the utility of the new approach to assess fungal denitrification potential in fungal isolates and agricultural soils. These new PCR primers may find application in a variety of biomes to assess the fungal contributions to N loss and N2O emissions.

Keyphrases

climate change
heavy metals
human health
risk assessment
nitric oxide
cell wall
healthcare
primary care
real time pcr
gene expression
wastewater treatment
dna methylation