Direct detection of iron clusters in L ferritins through ESI-MS experiments.
Lara MassaiSilvia CiambellottiLucrezia CosottiniLuigi MessoriPaola TuranoAlessandro PratesiPublished in: Dalton transactions (Cambridge, England : 2003) (2021)
Human cytoplasmic ferritins are heteropolymers of H and L subunits containing a catalytic ferroxidase center and a nucleation site for iron biomineralization, respectively. Here, ESI-MS successfully detected labile metal-protein interactions revealing the formation of tetra- and octa-iron clusters bound to L subunits, as previously underscored by X-ray crystallography.