Analysis of Metabolites in White Flowers of Magnolia Denudata Desr. and Violet Flowers of Magnolia Liliiflora Desr.
Chang Ha ParkSoo-Yun ParkSook Young LeeJae Kwang KimSang Un ParkPublished in: Molecules (Basel, Switzerland) (2018)
A total of seven phenolics and 44 metabolites was profiled in white flowers of Magnolia denudata and violet flowers of Magnolia liliiflora using high-performance liquid chromatography (HPLC), electrospray ionization-mass spectrometry (ESI-MS), and gas chromatography time-of-flight mass spectrometry (GC-TOFMS). Seven phenylpropanoid compounds were identified in white flowers by liquid chromatography mass spectrometry (LC-MS). An HPLC analysis showed that phenylpropanoid accumulation in violet flowers was 1.48 times higher than that in white flowers. Furthermore, superoxide dismutase (SOD)-like activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were determined to investigate the antioxidant properties of secondary metabolites in different flowers. Violet flowers showed higher SOD-like and DPPH activity than white flowers. In addition, anti-inflammatory activity measured using a nitric oxide assay was higher in violet flowers than in white flowers. Our results provide valuable information on the relationship between primary and secondary metabolites, and synergistic antioxidant and anti-inflammatory properties derived from phenolic compounds in different colored flowers.
Keyphrases
- mass spectrometry
- ms ms
- high performance liquid chromatography
- liquid chromatography
- gas chromatography
- tandem mass spectrometry
- nitric oxide
- anti inflammatory
- simultaneous determination
- solid phase extraction
- high resolution mass spectrometry
- healthcare
- high resolution
- hydrogen peroxide
- multiple sclerosis
- gas chromatography mass spectrometry
- amyotrophic lateral sclerosis
- single cell