A Turn-On Fluorescent Probe for Highly Selective Detection and Visualization of Hydrogen Sulfide in Fungi.
Qingsong YanShengui HeLei FengMing ZhangChaoyan HanYuzhuo WuChao WangXiaochi MaTonghui MaPublished in: Molecules (Basel, Switzerland) (2024)
Hydrogen sulfide (H 2 S) is a significant actor in the virulence and pathogenicity of fungi. The analysis of endogenous H 2 S in fungi benefits the prevention and treatment of pathogenic infections. Herein, a H 2 S-activated turn-on fluorescent probe named DDX-DNP was developed for the sensitive and selective detection of H 2 S. Using DDX-DNP , the ability of several oral fungi strains to produce H 2 S was identified, which was also validated using a typical chromogenic medium. In addition, DDX-DNP was successfully used for the visual sensing of endogenous H 2 S in fungal cells via microscope, flow cytometry, and colony imaging, along with a specific validation with the co-incubation of H 2 S production inhibitors in living cells. Above all, DDX-DNP could be used for H 2 S detection, the fluorescent imaging of fungi, and even the identification of related fungi.
Keyphrases
- living cells
- fluorescent probe
- flow cytometry
- single molecule
- high resolution
- escherichia coli
- loop mediated isothermal amplification
- label free
- real time pcr
- staphylococcus aureus
- pseudomonas aeruginosa
- biofilm formation
- cell death
- oxidative stress
- cell proliferation
- cell cycle arrest
- antimicrobial resistance
- signaling pathway
- cystic fibrosis
- candida albicans
- photodynamic therapy