Programming Cell Adhesion for On-Chip Sequential Boolean Logic Functions.
Xiangmeng QuShaopeng WangZhilei GeJianbang WangGuangbao YaoJiang LiXiaolei ZuoJiye ShiShiping SongLihua WangLi LiHao PeiChun-Hai FanPublished in: Journal of the American Chemical Society (2017)
Programmable remodelling of cell surfaces enables high-precision regulation of cell behavior. In this work, we developed in vitro constructed DNA-based chemical reaction networks (CRNs) to program on-chip cell adhesion. We found that the RGD-functionalized DNA CRNs are entirely noninvasive when interfaced with the fluidic mosaic membrane of living cells. DNA toehold with different lengths could tunably alter the release kinetics of cells, which shows rapid release in minutes with the use of a 6-base toehold. We further demonstrated the realization of Boolean logic functions by using DNA strand displacement reactions, which include multi-input and sequential cell logic gates (AND, OR, XOR, and AND-OR). This study provides a highly generic tool for self-organization of biological systems.
Keyphrases
- cell adhesion
- single molecule
- circulating tumor
- single cell
- living cells
- cell free
- cell therapy
- high throughput
- circulating tumor cells
- oxidative stress
- induced apoptosis
- nucleic acid
- mass spectrometry
- fluorescent probe
- wastewater treatment
- staphylococcus aureus
- stem cells
- cystic fibrosis
- cell proliferation
- sensitive detection