Bioactive Alkaloids from the Actinomycete Actinoalloteichus sp. ZZ1866.
Le QinWenwen YiXiao-Yuan LianZhizhen ZhangPublished in: Journal of natural products (2020)
The new alkaloids marinacarbolines E-Q (1-10, 12-14), caerulomycin N (15), and actinoallonaphthyridine A (16), together with the known marinacarboline C (11) and cyanogramide (17), were isolated from the actinomycete Actinoalloteichus sp. ZZ1866. The structures of the isolated compounds were elucidated based on their HRESIMS data, extensive NMR spectroscopic analyses, Mosher's method, ECD calculations, single-crystal X-ray diffraction analysis, and chemical degradation studies. Marinacarbolines E-L (1-8) share an indole-pyridone-imidazole tetracyclic skeleton, which is the first example of this kind of skeleton. Caerulomycin N (15) and cyanogramide (17) exhibited cytotoxic activity against both human glioma U251 and U87MG cells with IC50 values of 2.0-7.2 μM. Marinacarbolines E (1), G (3), I (5), and M (9) showed cytotoxic activity against U87MG cells with IC50 values of 2.3-8.9 μM.
Keyphrases
- induced apoptosis
- cell cycle arrest
- high resolution
- endothelial cells
- magnetic resonance
- endoplasmic reticulum stress
- signaling pathway
- oxidative stress
- magnetic resonance imaging
- molecular dynamics simulations
- molecular docking
- cell proliferation
- pi k akt
- computed tomography
- solid state
- density functional theory
- big data
- crystal structure