Construction of engineered 3D islet micro-tissue using porcine decellularized ECM for the treatment of diabetes.
Xiaocheng WangLijuan JinWenyu LiuLukas StingelinPan ZhangYanxiang ChengPublished in: Biomaterials science (2023)
Islet transplantation improves diabetes patients' long-term blood glucose control, but its success and utility are limited by cadaver availability, quality, and considerable islet loss after transplantation due to ischemia and inadequate angiogenesis. This study used adipose, pancreatic, and liver tissue decellularized extracellular matrix (dECM) hydrogels in an effort to recapitulate the islet sites inside the pancreas in vitro , and successfully generated viable and functional heterocellular islet micro-tissues using islet cells, human umbilical vein endothelial cells, and adipose-derived mesenchymal stem cells. The three-dimensional (3D) islet micro-tissues maintained prolonged viability and normal secretory function, and showed high drug sensitivity in drug testing. Meanwhile, the 3D islet micro-tissues significantly enhanced survival and graft function in a mouse model of diabetes. These supportive 3D physiomimetic dECM hydrogels can be used not only for islet micro-tissue culture in vitro , but also have great promise for islet transplantation for the treatment of diabetes.
Keyphrases
- extracellular matrix
- type diabetes
- glycemic control
- endothelial cells
- blood glucose
- cardiovascular disease
- mouse model
- gene expression
- adipose tissue
- emergency department
- end stage renal disease
- newly diagnosed
- induced apoptosis
- machine learning
- stem cells
- vascular endothelial growth factor
- cell death
- deep learning
- peritoneal dialysis
- combination therapy
- hyaluronic acid
- artificial intelligence
- cell proliferation
- electronic health record