Absolute quantitation of human wild-type DNAI1 protein in lung tissue using a nanoLC-PRM-MS-based targeted proteomics approach coupled with immunoprecipitation.
Hui WangXiaoyan NiNicholas ClarkKristen RandallLianne BoeglinSudha ChivukulaCaroline WooFrank DeRosaGang SunPublished in: Clinical proteomics (2024)
The results clearly demonstrate that the developed assay can accurately quantitate low-abundance WT DNAI1 protein in human lung tissue with high sensitivity, indicating its high potential use in the drug development for DNAI1 mutation-caused PCD therapy.
Keyphrases
- mass spectrometry
- wild type
- ms ms
- endothelial cells
- protein protein
- multiple sclerosis
- amino acid
- high throughput
- liquid chromatography
- binding protein
- liquid chromatography tandem mass spectrometry
- stem cells
- cancer therapy
- small molecule
- antibiotic resistance genes
- label free
- cell therapy
- wastewater treatment
- simultaneous determination
- solid phase extraction
- replacement therapy
- high throughput sequencing