Intrinsic blinking of red fluorescent proteins for super-resolution microscopy.

Natalia V KlementievaAnton I PavlikovAlexander A MoiseevNina G BozhanovaNatalie M MishinaSergey A LukyanovElena V ZagaynovaKonstantin A LukyanovAlexander S Mishin

Published in: Chemical communications (Cambridge, England) (2018)

Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.

Keyphrases

single molecule
living cells
quantum dots
atomic force microscopy
induced apoptosis
deep learning
machine learning
label free
cell cycle arrest
fluorescent probe
high resolution
high speed
signaling pathway
oxidative stress
cell proliferation
pi k akt